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stemmacs passaging solution xf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec stemmacs passaging solution xf
    Stemmacs Passaging Solution Xf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passaging/StemMACS+Passaging+Solution+XF%2C+human/us12686853-568-8-12
    Average 95 stars, based on 35 article reviews
    stemmacs passaging solution xf - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Passaging:

    Article Title: Oropouche virus infects human neural progenitor cells and alters the growth of brain organoids
    Article Snippet: StemMACS TM iPS-Brew XF , Miltenyi Biotec , Cat# 130-104-368. .. StemMACS Passaging Solution XF , Miltenyi Biotec , Cat# 130-104-688. ..

    Article Title: Methods and compositions for treatment of muscle disease with iPSC-induced human skeletal muscle stem cells
    Article Snippet: Isolated iPSC clones were confirmed to be OCT4 positive and Sendai virus negative. iPSCs were routinely maintained on Matrigel® (Corning) with StemMACS iPS-Brew XF® (Miltenyi Biotec). .. The cells were passaged every 4 days using StemMACS Passaging Solution XF® (Miltenyi Biotec) and the Rock inhibitor Y27632 (10 μM, STEMCELL Technologies) for 24 hr to improve the survival rates. .. iPSC typically plated on 6-well plates, were induced for myogenic differentiation using media prepared by Genea Biocells and now commercially available as a skeletal muscle differentiation kit with SKM03 S3 Stage differentiation medium (Myocea) following the manufacturer's specifications.

    Article Title: Cytosine base editing workflow for quality-controlled multiplex-knockout hiPSC lines
    Article Snippet: .. For hiPSCs, on the third day after the last passaging or when the hiPSCs reached more than 90% confluency, cells were seeded onto a 6-well plate for transfection (300,000 cells per well) using StemMACS Passaging Solution XF (Miltenyi Biotec). .. During seeding, 2.5 μM StemMACS Y-27632 (Miltenyi Biotec) was added until proceeding with the transfection.

    Article Title: Non-destructive transcriptomics via vesicular export
    Article Snippet: .. At ~70% confluence, hiPSCs were passaged using StemMACS Passaging Solution XF (Miltenyi Biotec) or Accutase (Gibco) at room temperature for 5 min, followed by manual dissociation in E8 Flex medium. .. Murine primary neurons (Gibco Thermo Fisher Scientific, A15586) were recovered according to the manufacturer’s protocol and maintained on PDL-coated (100 μg/mL) plates.

    Transfection:

    Article Title: Cytosine base editing workflow for quality-controlled multiplex-knockout hiPSC lines
    Article Snippet: .. For hiPSCs, on the third day after the last passaging or when the hiPSCs reached more than 90% confluency, cells were seeded onto a 6-well plate for transfection (300,000 cells per well) using StemMACS Passaging Solution XF (Miltenyi Biotec). .. During seeding, 2.5 μM StemMACS Y-27632 (Miltenyi Biotec) was added until proceeding with the transfection.

    other:

    Article Title: Oropouche virus infects human neural progenitor cells and alters the growth of brain organoids
    Article Snippet: Hamster BSR-T7/5 cells (Baby hamster kidney fibrobalst; gift of Alain Kohl, MRC-University of Glasgow, Scotland), which stably express the T7 RNA polymerase, were grown in GMEM supplemented with 10% (FBS), 1% P/S, 10% tryptose phosphate broth (Thermo Fisher Scientific), 1% GlutaMAX and Geniticin (1 mg/mL).

    Article Title: A chimeric human-mouse lung vascular model using induced pluripotent stem cells reveals insights into the pathogenesis of BMPR2 -related pulmonary hypertension
    Article Snippet: After 24 hours, the medium was replaced with 1mL/well of Phase 1 medium: STEMdiff TM APEL TM 2 Medium (STEMCELL Technologies, #05275), 25ng/mL rhBMP4 (R&D Systems, #314-BP), 50ng/mL rhFGF2 (R&D Systems, #233-FB), and 6uM CHIR99021 (Tocris, #4423).



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